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Cooling bath

A cooling bath or ice bath, in laboratory chemistry practice, is a liquid mixture which is used to maintain low temperatures, typically between 13 Ã‚°C and −196 Ã‚°C. These low temperatures are used to collect liquids after distillation, to remove solvents using a rotary evaporator, or to perform a chemical reaction below room temperature (see Kinetic control).

Cooling baths are generally one of two types: (a) a cold fluid (particularly liquid nitrogen, water, or even air) — but most commonly the term refers to (b) a mixture of 3 components: (1) a cooling agent (such as dry ice or ice); (2) a liquid "carrier" (such as liquid water, ethylene glycol, acetone, etc.), which transfers heat between the bath and the vessel; (3) an additive to depress the melting point of the solid/liquid system.

A familiar example of this is the use of an ice/rock-salt mixture to freeze ice cream. Adding salt lowers the freezing temperature of water, lowering the minimum temperature attainable with only ice.

Mixed-solvent cooling baths

Mixing solvents creates cooling baths with variable freezing points. Temperatures between approximately −78 Ã‚°C and −17 Ã‚°C can be maintained by placing coolant into a mixture of ethylene glycol and ethanol, while mixtures of methanol and water span the −128 Ã‚°C to 0 Ã‚°C temperature range. Dry ice sublimes at −78 Ã‚°C, while liquid nitrogen is used for colder baths.

As water or ethylene glycol freeze out of the mixture, the concentration of ethanol/methanol increases. This leads to a new, lower freezing point. With dry ice, these baths will never freeze solid, as pure methanol and ethanol both freeze below −78 Ã‚°C (−98 Ã‚°C and −114 Ã‚°C respectively).

Relative to traditional cooling baths, solvent mixtures are adaptable for a wide temperature range. In addition, the solvents necessary are cheaper and less toxic than those used in traditional baths.

Traditional cooling baths

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Water and ice baths

A bath of ice and water will maintain a temperature 0&nbsp;°C, since the melting point of water is 0&nbsp;°C. However, adding a salt such as sodium chloride will lower the temperature through the property of freezing-point depression. Although the exact temperature can be hard to control, the weight ratio of salt to ice influences the temperature:

  • −10&nbsp;°C can be achieved with a 1:2.5 mass ratio of calcium chloride hemihydrate to ice.
  • −20&nbsp;°C can be achieved with a 1:3 mass ratio of sodium chloride to ice.

Dry ice baths at −78 °C

Since dry ice will sublime at −78&nbsp;°C, a mixture such as acetone/dry ice will maintain −78&nbsp;°C. Also, the solution will not freeze because acetone requires a temperature of about −93&nbsp;°C to begin freezing.

Safety recommendations

The American Chemical Society notes that the ideal organic solvents to use in a cooling bath have the following characteristics:

  1. Nontoxic vapors.
  2. Low viscosity.
  3. Nonflammability.
  4. Low volatility.
  5. Suitable freezing point.

In some cases, a simple substitution can give nearly identical results while lowering risks. For example, using dry ice in 2-propanol rather than acetone yields a nearly identical temperature but avoids the volatility of acetone (see below).

See also

References

Further reading

External links