Citramalic acid is the organic compound with the formula HO<sub>2</sub>CCH<sub>2</sub>C(CH<sub>3</sub>)(OH)CO<sub>2</sub>H. A chiral compound, it is related structurally to malic acid.
Citramalic acid is the hydrated derivative of mesaconic acid, The hydration is catalyzed by mesaconyl-C4-CoA hydratase:
The same conversion can be achieved in vitro.
The enzyme (S)-citramalyl-CoA lyase converts citramalyl-CoA to acetyl-CoA and pyruvate.