In enzymology, a biuret amidohydrolase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are biuret and H<sub>2</sub>O, whereas its 3 products are urea, CO<sub>2</sub>, and NH<sub>3</sub>.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is biuret amidohydrolase. This enzyme participates in atrazine degradation.