In enzymology, a beta-aspartyl-N-acetylglucosaminidase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are 1-beta-aspartyl-N-acetyl-D-glucosaminylamine and H<sub>2</sub>O, whereas its two products are L-asparagine and N-acetyl-D-glucosamine.
This enzyme belongs to the family of hydrolases, specifically those glycosylases that hydrolyse N-glycosyl compounds. The systematic name of this enzyme class is 1-beta-aspartyl-N-acetyl-D-glucosaminylamine L-asparaginohydrolase. This enzyme is also called beta-aspartylacetylglucosaminidase.