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Aspartate—ammonia ligase (ADP-forming)

In enzymology, an aspartate—ammonia ligase (ADP-forming) () is an enzyme that catalyzes the chemical reaction

ATP + L-aspartate + NH<sub>3</sub> ADP + phosphate + L-asparagine

The 3 substrates of this enzyme are ATP, L-aspartate, and NH<sub>3</sub>, whereas its 3 products are ADP, phosphate, and L-asparagine.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-ammonia (or amine) ligases (amide synthases). The systematic name of this enzyme class is L-aspartate:ammonia ligase (ADP-forming). Other names in common use include asparagine synthetase (ADP-forming), and asparagine synthetase (adenosine diphosphate-forming). This enzyme participates in nitrogen metabolism.

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