Amorpha-4,11-diene 12-monooxygenase (, CYP71AV1) is an enzyme with systematic name amorpha-4,11-diene,NADPH:oxygen oxidoreductase (12-hydroxylating). This enzyme catalyses the following chemical reaction
amorpha-4,11-diene + 3 O<sub>2</sub> + 3 NADPH + 3 H<sup>+</sup> artemisinate + 3 NADP<sup>+</sup> + 4 H<sub>2</sub>O (overall reaction)
(1a) amorpha-4,11-diene + O<sub>2</sub> + NADPH + H<sup>+</sup> artemisinic alcohol + NADP<sup>+</sup> + H<sub>2</sub>O
(1b) artemisinic alcohol + O<sub>2</sub> + NADPH + H<sup>+</sup> artemisinic aldehyde + NADP<sup>+</sup> + 2 H<sub>2</sub>O
(1c) artemisinic aldehyde + O<sub>2</sub> + NADPH + H<sup>+</sup> artemisinate + NADP<sup>+</sup> + H<sub>2</sub>O
Amorpha-4,11-diene 12-monooxygenase is a heme-thiolate protein (P-450)
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