In enzymology, an aminoglycoside N6'-acetyltransferase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are acetyl-CoA and kanamycin B, whereas its two products are CoA and N6'-acetylkanamycin-B.
This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:kanamycin-B N6'-acetyltransferase. Other names in common use include aminoglycoside 6'-N-acetyltransferase, aminoglycoside-6'-acetyltransferase, aminoglycoside-6-N-acetyltransferase, and kanamycin acetyltransferase.
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes , , , and .