In enzymology, an allophanate hydrolase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are allophanate (urea-1-carboxylate or N-carbamoylcarbamate) and H<sub>2</sub>O, whereas its two products are HCO<sub>3</sub><sup>âÂÂ</sup> and NH<sub>4</sub><sup>+</sup>.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is urea-1-carboxylate amidohydrolase. This enzyme is also called allophanate lyase. This enzyme participates in urea cycle and metabolism of amino groups and atrazine degradation.