Aldos-2-ulose dehydratase (, pyranosone dehydratase, AUDH, 1,5-anhydro-<small>D</small>-fructose dehydratase (microthecin-forming)) is an enzyme with systematic name 1,5-anhydro-<small>D</small>-fructose hydro-lyase (microthecin-forming). This enzyme catalyses the following chemical reaction
1,5-anhydro-<small>D</small>-fructose 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one + H<sub>2</sub>O (overall reaction)
(1a) 1,5-anhydro-<small>D</small>-fructose 1,5-anhydro-4-deoxy-<small>D</small>-glycero-hex-3-en-2-ulose + H<sub>2</sub>O
(1b) 1,5-anhydro-4-deoxy-<small>D</small>-glycero-hex-3-en-2-ulose 2-hydroxy-2-(hydroxymethyl)-2H-pyran-3(6H)-one
This enzyme catalyses two of the steps in the anhydrofructose pathway.
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