In enzymology, an adenylylsulfatase () is an enzyme that catalyzes the chemical reaction
<div align=center>adenylyl sulfate + H<sub>2</sub>O AMP + sulfate + 2H<sup>+</sup></div>
Thus, the two substrates of this enzyme are adenylyl sulfate and H<sub>2</sub>O, whereas its two products are AMP and sulfate.
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in sulfonyl-containing anhydrides. The systematic name of this enzyme class is adenylyl-sulfate sulfohydrolase. Other names in common use include adenosine 5-phosphosulfate sulfohydrolase, and adenylylsulfate sulfohydrolase. This enzyme participates in sulfur metabolism.