In enzymology, acyl-CoA dehydrogenase (NADP+) () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are an acyl-CoA and oxidised nicotinamide adenine dinucleotide phosphate (NADP<sup>+</sup>). Its products are the corresponding 2,3-dehydroacyl-CoA, reduced NADPH, and a proton. The enzyme does not alter the length of the chain attached to coenzyme A but creates a double bond adjacent to the carbonyl group.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is acyl-CoA:NADP+ 2-oxidoreductase. Other names in common use include 2-enoyl-CoA reductase, dehydrogenase, acyl coenzyme A (nicotinamide adenine dinucleotide, phosphate), enoyl coenzyme A reductase, crotonyl coenzyme A reductase, crotonyl-CoA reductase, and acyl-CoA dehydrogenase (NADP+).
As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .