In enzymology, an acetylpyruvate hydrolase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are acetylpyruvate and H<sub>2</sub>O, whereas its two products are acetate and pyruvate.
This enzyme belongs to the family of hydrolases, specifically those acting on carbon-carbon bonds in ketonic substances. The systematic name of this enzyme class is 2,4-dioxopentanoate acetylhydrolase.