Yfr2 (cyanobacterial functional RNA-2 later published as Cyano-1 RNA motif) is a family of non-coding RNAs. Members of the Yrf2 family have been identified in almost all studied species of cyanobacteria. The family was identified through a bioinformatics screen of published cyanobacterial genomes, having previously been grouped in a family of Yfr2âÂÂ5.
The cyano-1 RNA motif family is essentially a synonym for Yfr2, although cyano-1 does not include the terminator sequence and Yfr2 does.
The function of this ncRNA is unknown, though it has been hypothesised that family members interact with RNA targets through kissing complexes using their highly conserved exposed central consensus element (CSE: IUPAC 5â²-RKT SGA AAC WHG GHM ASA M-3â²). Earlier studies noted a similarity to quorum sensing ncRNAs in Vibrio bacteria.
The family can be divided into two subgroups, one found in marine cyanobacteria (e.g. the Prochlorococcus and Synechococcus genera) and the other from freshwater cyanobacteria. Both subgroups have lengths of 63âÂÂ100 nucleotides (with the marine subgroup genes being on average 22nt longer), a conserved 5â² region and the characteristic Yfr2 CSE. Their secondary structures differ, however, with Yfr2 from marine picocyanobacteria having an additional stem loop. The position of Yfr2 genes is generally unconserved, but yfr2a orthologs were identified as being located 3â² to the gene 50S ribosomal subunit gene in almost all marine cyanobacteria studied. In the genus Prochlorococcus, Yfr2 genes are found adjacent to Yfr10 and a potential interaction between the two ncRNAs has been theorised.
The initial bioinformatics scan which identified yfr2 also identified yfr1, a functional RNA which is upregulated during stress conditions. Yfr families have now been identified up to yfr21.