Wyoming Highway 331 (WYO 331) is a east-west state highway in central Sheridan County, Wyoming, United States. It connects Sheridan County Route 53 (CR 53) in Beckton with Interstate 90 Business / U.S. Route 14 Business / U.S. Route 87 (I-90 BL / US 14 Bus. / US 87) in Sheridan.
WYO 331 begins at the north end of CR 53 (Big Goose Canyon Road) on the north bank of Big Goose Creek in the unincorporated community of Beckton. From its western terminus, WYO 331 heads briefly north on Big Goose Canyon Road before turning to head east-northeast, roughly paralleling the course of the Big Goose Creek. After heading east-northeast for roughly , WYO 331 reaches the outer limits of the city (and county seat) of Sheridan and becomes West Louks Street. After continuing northeast for about into Sheridan, it turns due east along West Louks Street. Approximately farther, it reaches its eastern terminus at I-90 BL / US 14 / US 87 (Main Street) in downtown Sheridan. (East Louks Street continues east from the eastern terminus.)