Vinnytsia Regiment (), originally known as Kalnyk Regiment (), was an administrative division of the Cossack Hetmanate. Its administrative headquarters was initially based in Kalnyk (modern-day Vinnytsia Oblast), but later transferred to Vinnytsia.
The regiment was mentioned in the Registry of the Zaporozhian Host compiled following the Treaty of Zboriv in 1649. In 1669 the regiment's Cossacks mutinied against hetman Petro Doroshenko, supporting his Zaporozhian Sich rival Petro Sukhoviy. After Doroshenko's fall from popularity, in 1675 the regiment recognized the rule of Polish-supported acting hetman Ostap Hohol. The regiment remained in the Polish sphere of influence until 1704. In 1711 the regiment's Cossacks joined the forces of Pylyp Orlyk during his campaign against the troops of Peter I of Russia; as a result, after Peter's counteroffensive they were deported to the Left-bank Ukraine.
According to the 1649 registry, the regiment consisted of the following subdivisions: