Villa Estense is a comune (municipality) in the Province of Padua in the Italian region Veneto, located about southwest of Venice and about southwest of Padua. As of 31 December 2004, it had a population of 2,407 and an area of .
Villa Estense borders the following municipalities: Este, Granze, Sant'Elena, Sant'Urbano, Vescovana, Vighizzolo d'Este.