The Apostolic Vicariate (or Vicariate Apostolic) of Puerto Ayacucho () is a Latin Church missionary ecclesiastical jurisdiction or apostolic vicariate of the Catholic Church in Venezuela.
Its cathedral see, Catedral MarÃÂa Auxiliadora, is located in the town of Puerto Ayacucho, in Venezuela's Amazonas state. It is immediately exempt to the Holy See and not part of any ecclesiastical province.
On 5 February 1932 Pope Pius XI established the Prefecture Apostolic of Alto Orinoco from territory taken from the then Diocese of Santo Tomás de Guayana (which meanwhile became the Metropolitan Archdiocese of Ciudad BolÃÂvar).
It was elevated to a Vicariate Apostolic and given its present name by Pope Pius XII on 7 May 1953.
So far, all incumbents have been members of the missionary Salesians (S.D.B.) congregation