Uzbekistan competed at the 2014 Summer Youth Olympics, in Nanjing, China from 16 August to 28 August 2014.
Uzbekistan qualified three boxers based on its performance at the 2014 AIBA Youth World Championships
Uzbekistan qualified one boat based on its performance at the 2013 World Junior Canoe Sprint and Slalom Championships.
Uzbekistan qualified two athletes based on its performance at the 2014 Asian Artistic Gymnastics Championships.
Uzbekistan qualified one individual and one team based on its performance at the 2014 Asian Rhythmic Championships.
Uzbekistan qualified one athlete based on its performance at the 2014 Asian Trampoline Championships.
Uzbekistan qualified one athlete based on its performance at the 2013 Cadet World Judo Championships.
Uzbekistan qualified two boats based on its performance at the Asian Qualification Regatta.
<small>Qualification Legend: FA=Final A (medal); FB=Final B (non-medal); FC=Final C (non-medal); FD=Final D (non-medal); SA/B=Semifinals A/B; SC/D=Semifinals C/D; R=Repechage</small>
Uzbekistan qualified two shooters based on its performance at the 2014 Asian Shooting Championships.
Uzbekistan qualified two swimmers.
Uzbekistan qualified one athlete based on its performance at the Asian qualifying event.
<small>Qualification Legend: Q=Main Bracket (medal); qB=Consolation Bracket (non-medal)</small>
Uzbekistan qualified one athlete based on its performance at the Taekwondo Qualification Tournament.
Uzbekistan qualified 2 quotas in the boys' events based on the team ranking after the 2013 Weightlifting Youth World Championships. Later Uzbekistan would qualify 1 quota in the girls' events based on the team ranking after the 2014 Weightlifting Youth & Junior Asian Championships.
Uzbekistan qualified three athletes based on its performance at the 2014 Asian Cadet Championships.