Tunisia competed at the 2014 Summer Youth Olympics, in Nanjing, China from 16 August to 28 August 2014.
Tunisia qualified six athletes.
<small>Qualification Legend: Q=Final A (medal); qB=Final B (non-medal); qC=Final C (non-medal); qD=Final D (non-medal); qE=Final E (non-medal)</small>
Tunisia qualified a boys' team based on the 1 June 2014 FIBA 3x3 National Federation Rankings.
Tunisia qualified one boxer based on its performance at the 2014 AIBA Youth World Championships
Tunisia qualified one athlete based on its performance at the 2014 FIE Cadet World Championships.
Tunisia qualified one athlete based on its performance at the 2014 African Artistic Gymnastics Championships.
Tunisia qualified one team based on its performance at the 2014 African Men's Youth Handball Championship.
Tunisia qualified one athlete based on its performance at the 2013 Cadet World Judo Championships.
Tunisia qualified two boats based on its performance at the African Qualification Regatta.
<small>Qualification Legend: FA=Final A (medal); FB=Final B (non-medal); FC=Final C (non-medal); FD=Final D (non-medal); SA/B=Semifinals A/B; SC/D=Semifinals C/D; R=Repechage</small>
Tunisia qualified a girls' team based on its performance at the 2013 Rugby World Cup Sevens.
Tunisia qualified one boat based on its performance at the Techno 293 African Continental Qualifiers.
Tunisia qualified two swimmers.
Tunisia qualified one athlete based on its performance at the African Qualification Event.
<small>Qualification Legend: Q=Main Bracket (medal); qB=Consolation Bracket (non-medal)</small>
Tunisia qualified 1 quota in the boys' and girls' events based on the team ranking after the 2014 Weightlifting Junior & Youth African Championships.
Tunisia qualified two athletes based on its performance at the 2014 African Cadet Championships.