The Tuanku Ja'afar Hospital (HTJS; ) is a government funded district general hospital in Seremban, Negeri Sembilan, Malaysia. It is the largest hospital in Negeri Sembilan.
The construction of the hospital began in 1969 and went into operation in 1972 as Seremban General Hospital. On 27 July 2006, the hospital was renamed as Tuanku Ja'afar Hospital.
Tuanku Ja'afar Hospital has 1,070 beds and is the largest hospital in Negeri Sembilan.