is a passenger railway station located in the city of Tsuyama, Okayama Prefecture, Japan, operated by West Japan Railway Company (JR West).
Tsuboi Station is served by the Kishin Line, and is located 98.3 kilometers from the southern terminus of the line at .
The station consists of two opposed ground-level side platforms connected to the station building by a level crossing. The station is unattended.
Tsuboi Station opened on August 21, 1923. With the privatization of the Japan National Railways (JNR) on April 1, 1987, the station came under the aegis of the West Japan Railway Company.
In fiscal 2019, the station was used by an average of 24 passengers daily.