Skotoussa (, ) is a village and a former municipality in the Serres regional unit, Greece. Population 3,248 (2021). Since the 2011 local government reform it is part of the municipality Irakleia, of which it is a municipal unit. The municipal unit has an area of 99.433 km<sup>2</sup>.
The settlement is served by Skotoussa railway station on the Thessaloniki-Alexandroupoli line, with daily services to Thessaloniki and Alexandroupolis.