Sirius Red

Sirius Red F 3B (Direct Red 80) is an azo dye primarily used in staining methods for collagen and amyloid. It has the molecular formula C₄₅H₂₆N₁₀Na₆O₂₁S₆.

In histology, sirius red staining is used in various domains of diagnostic to observe fibrosis levels in a lot of cases of inflammation induced by cancer, vascular or metabolic pathologies.

In bright field microscopy the following can be observed:

• The nuclei in yellow
• The cytoplasm in yellow
• Collagen fibers in red
• Muscular fibers in yellow
• Red blood cells in yellow

See also

• Collagen Hybridizing Peptide, a peptide that stains denatured collagen in tissues

References