The Shire of Healesville was a local government area about northeast of Melbourne, the state capital of Victoria, Australia. The shire covered an area of , and existed from 1887 until 1994.
Healesville was first incorporated as a shire on 30 September 1887. It annexed parts of the Shire of Yea on 21 April 1925, and the Christmas Hills area of the Shire of Eltham on 18 June 1958, while losing land to the Shire of Alexandra on two occasions, 1 October 1963 and 1 October 1984.
Originally the Shire of Healesville included considerable territory over the Great Dividing Range from Healesville itself. Buxton, Taggerty and Thornton were ceded to the Shire of Alexandra in 1963, while Marysville and Narbethong were not transferred until 1984.
On 15 December 1994, the Shire of Healesville was abolished, and along with the Shires of Lillydale and Upper Yarra, and parts of the Shire of Sherbrooke, was merged into the newly created Shire of Yarra Ranges.
From 1967, the Shire of Healesville was divided into four ridings:
At the time of its dissolution, it was divided into three ridings, each of which elected three councillors:
<small>* Council seat.</small>
<small>* Estimate in the 1958 Victorian Year Book.</small>