The Chamber of Senators of Santa Fe Province () is the upper house of the Legislature of Santa Fe Province, the third most populous of Argentina's provinces.
The entirety of its members are renewed every four years alongside the governor and the Chamber of Deputies, the lower house of the provincial legislature. It is made up of 19 senators who represent each of the province's 19 departments, each elected using the first-past-the-post system.
The first legislative body of Santa Fe Province was established in 1819 with the provincial statute, which granted attributions to the . A unicameral legislature, composed of 16 members, was established later on by the Constitution of Santa Fe of 1856.
A constitutional reform passed in 1872 during the governorship of Simón de Iriondo divided the legislature into an upper house, the Senate, and a lower house, the Chamber of Deputies, taking after the National Congress of Argentina.
Alongside the Chamber of Deputies, the Senate convenes in the former Customs building in the provincial capital of Santa Fe de la Vera Cruz.
The Senate is chaired by the vice governor of the province, who is elected alongside the governor every four years. The Vice Governor may only cast tie-breaking votes (according to article 39 of the provincial constitution). The following is a list of vice governors of Buenos Aires since the return of democracy in 1983.