Scytodes trifoliata is a species of spider in the family Scytodidae. It is endemic to South Africa and is commonly known as the Port Shepstone spitting spider.
Scytodes trifoliata is found in three South African provinces, KwaZulu-Natal (Port Shepstone), Eastern Cape, and Mpumalanga.
The species inhabits the Indian Ocean Coastal Belt, Savanna and Thicket biomes at altitudes ranging from 52 to 197 m above sea level. It is a wandering spider commonly collected from under stones and dark places on the soil surface.
Scytodes trifoliata is listed as Least Concern by the South African National Biodiversity Institute. Although the species is known only from females, it has a wide geographical range. The species is protected in two protected areas Cwebe Nature Reserve and Kruger National Park.
The species has not been revised and is known only from females.