Sant'Ambrogio di Valpolicella () is a comune (municipality) in the Province of Verona in the Italian region Veneto, located about west of Venice and about northwest of Verona.
Sant'Ambrogio di Valpolicella borders the following municipalities: Cavaion Veronese, Dolcè, Fumane, Pastrengo, Pescantina, Rivoli Veronese, and San Pietro in Cariano. Its frazione of San Giorgio is one of I Borghi più belli d'Italia ("The most beautiful villages of Italy").
Sights include the Romanesque pieve of San Giorgio, built in the 12th century over a Lombard (and perhaps pre-Roman) religious place. The interior has a nave and two aisles, of the same height, divided by piers. Some of the latter are decorated by 14th-century paintings. The basements of the columns are re-used Roman altars. The church houses also 11th-century frescoes, including a Last Supper; also notable is the ciborium, built during the reign of the Lombard king Liutprand (711-744). The bell tower and the cloister are also from the 12th century.
Sant'Ambrogio is twinned with: