Rotylenchulus is a genus of nematodes in the family Hoplolaimidae. They are mostly found in tropical and subtropical areas where they are semi-endoparasites of the roots of herbaceous and woody plants. Species are known to infest a wide range of plants including wheat, sunflowers and grapes.
Molecular characteristics provide important diagnostic tools, particularly since there is high intraspecific variability in immature females. The following species are recognised: