The Roman Catholic Diocese of San Juan Bautista (de las Misiones) () is a southern suffragan Latin Catholic diocese in the ecclesiastical province of Asunción, which covers all of Paraguay (except the pre-diocesan missions).
Its cathedral episcopal see is Catedral San Juan Bautista, dedicated to John the Baptist, in the city of San Juan Bautista de las Misiones in Misiones Department
It also has a Minor Basilica : the Marian BasÃÂlica Nuestra Señora del Pilar, dedicated to Our Lady of Pilar, in Pilar, Paraguay, ÃÂeembucu.
As per 2014, it pastorally served 227,000 Catholics (98.3% of 231,000 total) on 21,700 km<sup>2</sup> in 30 parishes and 139 missions with 35 priests (19 diocesan, 16 religious), 2 deacons, 63 lay religious (18 brothers, 45 sisters) and 5 seminarians.