The Roman Catholic Diocese of San Fernando de Apure () is a suffragan Latin diocese in the ecclesiastical province of Calabozo in Venezuela.
Its cathedral episcopal see is located in the city of San Fernando de Apure.
It was established on 7 June 1954 as Territorial Prelature of San Fernando de Apure, of territories split off from the Dioceses of Calabozo and San Cristóbal de Venezuela. It was promoted on 12 November 1974 as the Diocese of San Fernando de Apure. It lost territory on 3 December 2015 to establish (part of) the Diocese of Guasdualito.