The Diocese of Presidente Prudente () is a Latin Church ecclesiastical territory or diocese of the Catholic Church in Southern Brazil. It is a suffragan diocese in the ecclesiastical province of the metropolitan Archdiocese of Botucatu.
Its cathedra is in the Catedral São Sebastião, dedicated to Saint Sebastian, in the episcopal see of Presidente Prudente.
As per 2015, it pastorally served 542,400 Catholics (93.1% of 582,700 total) on 15,513 km<sup>2</sup> in 53 parishes and 4 missions with 67 priests (55 diocesan, 12 religious), 58 lay religious (22 brothers, 36 sisters) and 17 seminarians .
(all native Brazilians)