This is a list of rolling stock used by the Mid-Suffolk Light Railway.
The museum currently has six locomotives on site, three operational steam engines, and "1604" which is under restoration and owned by the MSLR. It also has two Ruston diesels; a 165 named "Alston" and a Ruston 48, which is very similar to the engine used to take up the railway.
The MSLR's coaching stock is entirely made up of ex-GER carriages, to replicate what the line's original stock would've been like many years ago.
There are also several wagons and freight items that are under restoration and operational.
Some signs of the original rolling stock is visible in the countryside surrounding the railway. One example is a semi-derelict carriage on farmland near Mendlesham at (). It may be seen on Google maps satellite view.
The railway owns a few vintage Great Eastern covered vans, one LNER van, a special LNER survivor; a 'Toad B' brake van No. 157787, due to the mass amount of vans and minuscule open wagons, the MSLR built a replica open wagon to a GER design in 2012.