Rajni Tiwari (born 21 July 1973) is an Indian politician, a Minister of State in the Government of Uttar Pradesh and a member of the 18th Legislative Assembly. She represents the Shahabad constituency and is a member of the Bhartiya Janta Party.
Tiwari was born on 21 July 1973 to Krishna Prasad Agnihotri in Bilgram in Hardoi district. She graduated with a Bachelor of Arts from Arya Kanya Degree College, Hardoi, Kanpur University. Tiwari is an agriculturalist and businesswoman by profession.
Tiwari was married to Upendra Tiwari, with whom she has a son and a daughter. Upendra Tiwari was an MLA from Bilgram constituency. He died in 2007 which left the Bilgram constituency seat vacant. Tiwari was elected as an MLA in the 2008 by-polls.