Pyin-O-Lwin Bird Sanctuary is a protected area in Myanmar's Pyinoolwin Township in Mandalay Region covering an area of . It was gazetted in 1918 and spans an elevation of . It is one of four protected wetlands.
This sanctuary is near the town of Pyin-O-Lwin. The road linking Anesakhan () and Lashio () towns passes through the sanctuary. It receives south-west monsoon rains of about every year from June to August.
Pyin-O-Lwin Bird Sanctuary harbours evergreen hill forest. Wildlife present include green peafowl (Pavo muticus), grey peacock pheasant (Polyplectron bicalcaratum) and barking deer (Muntiacus muntjak).
Pyin-O-Lwin Bird Sanctuary was gazetted in 1918. It was established in 1927. As of 2017, it is managed by the Forest Department.
Pyin-O-Lwin town is a favourite destination for the tourist since, it is located on the main road and railway line from Mandalay to Lashio. Many tourist attractions like National Kandawgyi Gardens (), flower garden have resulted in need to develop Pyin-O-Lwin Bird sanctuary. Regular educated forest staff is in need to direct the local and foreign tourist visiting the sanctuary.