tRNA pseudouridine<sup>13</sup> synthase (, TruD, YgbO, tRNA PSI13 synthase, RNA:PSI-synthase Pus7p, Pus7p, RNA:pseudouridine-synthase Pus7p, Pus7 protein) is an enzyme with systematic name tRNA-uridine<sup>13</sup> uracil mutase. This enzyme catalyses the following chemical reaction
Pseudouridine synthase TruD from Escherichia coli specifically acts on uridine<sup>13</sup> in tRNA.