Puerto Rico competed at the 2014 Summer Youth Olympics, in Nanjing, China from 16 August to 28 August 2014.
Puerto Rico qualified six athletes.
<small>Qualification Legend: Q=Final A (medal); qB=Final B (non-medal); qC=Final C (non-medal); qD=Final D (non-medal); qE=Final E (non-medal)</small>
Puerto Rico qualified a boys' team based on the 1 June 2014 FIBA 3x3 National Federation Rankings.
Puerto Rico qualified a boys' and girls' team by their performance at the NORCECA Final YOG Qualifier.
Puerto Rico qualified one athlete based on its performance at the 2014 FIE Cadet World Championships
Puerto Rico qualified two athletes based on its performance at the 2014 Junior Pan American Artistic Gymnastics Championships.
Puerto Rico qualified two athletes based on its performance at the 2013 Cadet World Judo Championships.
Puerto Rico was given a reallocation boat based on being a top ranked nation not yet qualified.
Puerto Rico qualified one swimmer.
Puerto Rico qualified one athlete based on its performance at the Latin American Qualification Event.
<small>Qualification Legend: Q=Main Bracket (medal); qB=Consolation Bracket (non-medal)</small>
Puerto Rico qualified one athlete based on its performance at the Taekwondo Qualification Tournament.