PreQ1-III riboswitches are a class of riboswitches that bind pre-queuosine<sub>1</sub> (PreQ<sub>1</sub>), a precursor to the modified nucleoside queuosine. PreQ<sub>1</sub>-III riboswitches are the third class of riboswitches to be discovered that sense this ligand, and are structurally distinct from preQ<sub>1</sub>-I and preQ<sub>1</sub>-II riboswitches. Most sequenced examples of preQ<sub>1</sub>-III riboswitches are obtained from uncultivated metagenome samples, but the few examples in cultivated organisms are present in strains that are known to or suspected to be Faecalibacterium prausnitzii, a species of Gram-positive Clostridia. Known examples of preQ<sub>1</sub>-III riboswitches are found upstream of queT genes, which are expected to encode transporters of a queuosine derivative. The other two known classes of preQ<sub>1</sub> riboswitches are also commonly found upstream of queT genes.
The atomic-resolution structure of a preQ1-III riboswitch has been solved by X-ray crystallography.