The Ponce and Guayama Railroad is an abandoned railroad in Puerto Rico, primarily used to transport products of a sugar mill in Ponce, Puerto Rico. Operating from 1910 to 1990, it was the last sugar mill railroad in Puerto Rico. The railroad used a one-meter track gauge and had a mainline track length of 30.4 miles.
Passenger service on the railroad was inaugurated on 1 October 1910. It was initially named American Railroad Co. of Porto Rico, Aguirre Operator.