Petukhovo () is an alkaline lake in Klyuchevsky District, Altai Krai, Russian Federation.
The lake is located at the western edge of the Krai. The Russia-Kazakhstan border lies to the southwest. The nearest inhabited places are Petukhi, close to the southern shore, and Makarovka, near the northwestern end. Klyuchi, the district capital, lies to the west.
In 1998 the lake was declared a natural monument of regional significance. Petukhovo is a soda lake and there is a soda plant operating near it that has been extracting raw material from it for over a century.
Located in the Kulunda Plain, Petukhovo has an irregular shape, stretching roughly from northwest to southeast for approximately . In the north and northwest the lake is bound by a to high shore, while the eastern and southeastern shores have up to high cliffs. Silt deposits at the bottom of the lake reach a thickness of in the central part. The minerals in the bottom sediments are dominated by terrigenous quartz, feldspars, excess-Ca dolomite, and Mg-calcite, with the proportion of carbonates increasing in the lower part of the section. The water has a mineralization of and a pH of 9.5.
Lake Shukyrtuz lies to the north and Kurichye to the south. to the southwest there is another smaller lake named Petukhovo or Petukhovskoye, west of Severka.
The lake is surrounded by deforested steppe landscape of low hills.