Ospedaletto Euganeo is a comune (municipality) in the province of Padua in the Italian region of Veneto, located about southwest of Venice and about southwest of Padua. As of 31 December 2004, it had a population of 5,667 and an area of .
The municipality of Ospedaletto Euganeo contains the frazioni (subdivisions, mainly villages and hamlets) Tresto, Palugana, Peagnola, Santa Croce, and Dossi.
Ospedaletto Euganeo borders the following municipalities: Carceri, Este, Lozzo Atestino, Noventa Vicentina, Ponso, Saletto, Santa Margherita d'Adige.