Novonikolayevka () is a rural locality (a village) in Tynbayevsky Selsoviet, Mishkinsky District, Bashkortostan, Russia. The population was 154 as of 2010. There are 3 streets.
Novonikolayevka is located 51 km northwest of Mishkino (the district's administrative centre) by road. Tynbayevo is the nearest rural locality.