Route 320 is a collector road in the Canadian province of Nova Scotia.
It is located in Richmond County and connects Louisdale at Exit 46 on Highway 104 with Arichat at Route 206 .
It crosses the Lennox Passage using the Burnt Island Bridge (a drawbridge) that connects Cape Breton Island to Isle Madame.
The section of Collector Highway 320 from Arichat to Harbourview Crescent was once designated as Trunk Highway 20.
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