NotI

NotI is a restriction enzyme isolated from the bacterium Nocardia otitidis.

Recognition sequence

Palindromic recognition sequence of NotI:

5'-GCGGCCGC-3' 3'-CGCCGGCG-5'

The ends generated by NotI digest:

5'---GC GGCCGC---3' 3'---CGCCGG CG---5'

References