Nikolaos Mitrou (; born 10 July 1984) is a Greek former professional footballer who played as a midfielder.
Born in Chalcis, Mitrou began his playing career by signing with Panionios F.C. in August 2002. He made 32 appearances in the Super League Greece for the club. He also played for Niki Volos, Digenis Akritas Morphou, Ethnikos Asteras, Lamia, Aias Salamina F.C., Kastoria, Pierikos, Enosi Mideas, Poros and FC Zenit ÃÂáslav.