In enzymology, a NAD<sup>+</sup> synthase (glutamine-hydrolysing) () is an enzyme that catalyzes the chemical reaction
The substrates of this enzyme are ATP, deamido-NAD+, L-glutamine, and H<sub>2</sub>O, whereas its 4 products are AMP, diphosphate, NAD<sup>+</sup>, and glutamate
This enzyme participates in glutamate metabolism and nicotinate and nicotinamide metabolism.
This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name of this enzyme class is deamido-NAD+:L-glutamine amido-ligase (AMP-forming).