N<sup>8</sup>-acetylspermidine oxidase (propane-1,3-diamine-forming) () is an enzyme with systematic name N<sup>8</sup>-acetylspermidine:oxygen oxidoreductase (propane-1,3-diamine-forming). This enzyme catalyses the following chemical reaction
This enzyme is also active n N<sup>1</sup>-acetylspermine, and it has weak activity on N<sup>1</sup>,N<sup>12</sup>-diacetylspermine.