The enzyme N-sulfoglucosamine-3-sulfatase (EC 3.1.6.15}) catalyzes cleaving off the 3-sulfate groups of the N-sulfo-<small>D</small>-glucosamine 3-O-sulfate units of heparin.
This enzyme belongs to the family of hydrolases, specifically those acting on sulfuric ester bonds. The systematic name is N-sulfo-3-sulfoglucosamine 3-sulfohydrolase. This enzyme is also called chondroitinsulfatase. This enzyme participates in the degradation of glycan structures.