In enzymology, an N-methylhydantoinase (ATP-hydrolysing) () is an enzyme that catalyzes the chemical reaction
The 3 substrates of this enzyme are ATP, N-methylimidazolidine-2,4-dione, and H<sub>2</sub>O, whereas its 3 products are ADP, phosphate, and N-carbamoylsarcosine.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is N-methylimidazolidine-2,4-dione amidohydrolase (ATP-hydrolysing). Other names in common use include N-methylhydantoin amidohydrolase, methylhydantoin amidase, N-methylhydantoin hydrolase, and N-methylhydantoinase. This enzyme participates in arginine, creatinine, and proline metabolism.