In enzymology, a N-carbamoylsarcosine amidase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are N-carbamoylsarcosine and H<sub>2</sub>O, whereas its 3 products are sarcosine, CO<sub>2</sub>, and NH<sub>3</sub>.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-carbamoylsarcosine amidohydrolase. This enzyme is also called carbamoylsarcosine amidase. This enzyme participates in arginine and proline metabolism.
As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .