In enzymology, a N-carbamoyl-L-amino-acid hydrolase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are N-carbamoyl-L-2-amino acid and H<sub>2</sub>O, whereas its 3 products are L-2-amino acid, NH<sub>3</sub>, and CO<sub>2</sub>.
This enzyme belongs to the subfamily of hydrolases which act on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-carbamoyl-L-amino acid amidohydrolase.